Journal: iScience

Article Title: 3D-printed scaffold loaded with baicalin exosomes promotes bone defect repair via mediating PRRX2 to alleviate inflammation

doi: 10.1016/j.isci.2025.113565

Figure Lengend Snippet: Identification and angiogenesis of exos from BA-pretreated BMSCs (A) The morphology of BMSC-exos and BA-BMSC-exos under transmission electron microscopy. (B) Nanoparticle tracking analysis showing the size distribution of BMSC-exos and BA-BMSC-exos. (C) The expression levels of the exosome markers CD9, TSG101, and CD81 were measured by western blot. (D) The uptake of BA-BMSC-exos by HUVECs was detected by immunofluorescence staining (scale bar: 100 μm). (E) CCK8 determined the viability of HUVECs after treatment with exos. (F) Cell migration of HUVECs determined by Transwell assay (scale bar: 100 μm). (G) Tube formation of HUVECs following treatment with exos (scale bar: 100 μm). The expression of VEGF and CD31 in HUVECs was determined by (H) Western blot and (I) qPCR. Data are presented as mean ± standard deviation (SD), n = 3, p -values are calculated using one-way or two-way ANOVA, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: After washing three times, the membranes were stained with primary antibodies against CD9, TSG101, CD31, p -AKT, AKT, IL-6, IL-1β, TNF-α, Nrf2, and HO-1 (all from Abcam, UK), vascular endothelial growth factor (VEGF) (from Proteintech, USA) overnight at 4 °C.

Techniques: Transmission Assay, Electron Microscopy, Expressing, Western Blot, Immunofluorescence, Staining, Migration, Transwell Assay, Standard Deviation